Fermentation as a scalable technology starts with the microorganism hosts that act as factories in which cells are capable of producing very precise ingredients through very rapid duplication. Fermenting proteins requires the synergy between protein of interest and microorganism strain. Different proteins, and even different classes of enzymes are known to be better suited to specific microorganisms. To achieve optimum fermentation expression of the protein, genetic engineering or evolutionary optimisation methods can be explored to best reach target strain productivity.
For the production of enzymes and proteins, we access prokaryotic host organisms (including Escherichia coli, Bacillus subtilis, Pseudomonas stutzeri, Streptomyces lividans and Corynebacterium glutamicum) and eukaryotic host organisms (including Pichia pastoris, Kluyveromyces lactis and Aspergillus niger). For the expression of a gene of interest, the gene can be inserted into an expression host via BRAIN’s engineered Cas nuclease BEC or classical methods utilizing different auxotrophic and antibiotic selection markers.
For high productivity of protein expression in fermentation, different microorganism strains are better suited to different proteins of interest. Aligning the protein of interest to a suitable microorganism strain is a step in the right direction of a commercially viable process and product. The proprietary Bioarchive can be screened to specific parameters (e.g. food-grade, GRAS, IDF, or China-listed microorganisms, including yeasts, fungi, LAB etc.) to identify a shortlist of suitable microorganism strains that can the be characterised by microbiological techniques as well as genome analysis and growth and performance tests under application-specific conditions.
For more information on microorganism discovery capabilities and technologies click here
We have the technologies and experience to support microorganism strain development from diverse genome editing methodologies (e.g. proprietary CRISPR-Cas) as well as adaptive laboratory evolution (ALE) techniques. Our technologies can be deployed to best create a highly individualised concept for strain development, in ensuring we best achieve the target strain productivity relevant to the protein of interest and market application.
Interested in learning more about our optimisation techniques, read more here.
All of our research and development work is planned and executed with scalability in mind. We know your innovations are destined for commercial applications and so we ensure at every step of the way we develop a process and product that can be reproduced at commercial volumes. At this stage we take the developed and optimised production strain that we know works on laboratory scale, and begin to scale up the microbial production process to pilot- and industrial scale. Using biology and engineering skills and broad knowledge of process development, we set up upstream and downstream processes in close collaboration with customers. For a process transfer, processes can be scaled and optimised up to 200 litres at our BRAIN R&D innovation centre in Zwingenberg, Germany, with additional larger scale capacities seamlessly transferred to our manufacturing and state-of-the-art fermentation facility in Cardiff, United Kingdom.
Click here for more information on scaling up at larger scales in our fermentation facility in the UK.